The Stratagene QuikChange® Multi Site-Directed Mutagenesis Kit (catalog #200514) instruction manual provides step‑by‑step protocols, primer design…
This manual describes the contents of the QuikChange® Multi Site-Directed Mutagenesis Kit, the required materials, storage conditions, and detailed three‑step mutagenesis protocol. It also includes guidelines for mutagenic primer design, transformation of XL10‑Gold® ultracompetent cells, and troubleshooting tips.
The kit supplies enzyme blend, reaction buffer, dNTP mix, Dpn I enzyme, control template and primers, and XL10‑Gold® cells, enabling rapid generation of multiple site‑directed mutations in a single reaction.
| Catalog number | 200514 |
|---|---|
| Enzyme blend concentration | 2.5 U/µl |
| Reaction buffer volume | 200 µl |
| QuikSolution volume | 500 µl |
| dNTP mix volume | 30 µl |
| Dpn I enzyme amount | 300 U |
| XL10‑Gold® cells aliquot | 135 µl per 10× |
| Storage temperature – cells & plasmid | –80 °C |
| Storage temperature – other components | –20 °C |
The kit is catalog #200514.
XL10‑Gold® ultracompetent cells and the control plasmid are stored at –80 °C, while all other components are stored at –20 °C.
The kit provides a proprietary enzyme blend featuring PfuTurbo® DNA polymerase.