QuikHyb Hybridization Solution INSTRUCTION MANUAL
The QuikHyb Hybridization Solution is designed to reduce the time required for Southern, Northern, slot-blot, and plaque-lift hybridizations from 12–24 hours down to 1–2 hours. It is suitable for use with randomly labeled radioactive and nonradioactive nucleic acid probes in various formats.
The document details the materials provided, storage conditions (store at 4°C), and step-by-step protocols for prehybridization, probe preparation, hybridization, washing, detection, and stripping membranes for reuse. It also includes appendices on calculating hybridization and melting temperatures.
| Molar concentration of NaCl | 0.5 M |
|---|---|
| Storage Temperature | 4°C |
The solution reduces the time required for hybridization from the customary 12–24 hours down to 1–2 hours.
Use a minimum of 33 μl of QuikHyb hybridization solution per cm² of the blot.
Prehybridize the membrane in QuikHyb solution at 68°C for 10–20 minutes.