This document is the instruction manual for the Lambda DNA Purification Kit, describing the features and characteristics of the product.
The Lambda DNA Purification Kit facilitates the rapid purification of high-grade lambda DNA by eliminating the need for toxic phenol-chloroform extraction and time-consuming polyethylene glycol precipitation steps. The kit utilizes diethylaminoethyl (DEAE) resin to remove contaminating polyanions prior to phage particle disruption.
| Catalog Numbers | #200391 and #200392 |
|---|---|
| DEAE-cellulose Volume (Cat #200391) | 25 ml |
| DNase I Concentration | 20 mg/ml |
| RNase A Concentration | 2 mg/ml |
| Pronase Concentration | 50 mg/ml |
RNase and DNase must be stored at -20°C.
The culture should be grown overnight with shaking at 30°C.