Instruction manual for StrataClone PCR Cloning Kit
The Stratagene StrataClone PCR Cloning Kit enables the efficient cloning of PCR products by utilizing a topoisomerase I-charged vector and Cre recombinase-expressing competent cells. The kit includes all necessary reagents for up to 21 reactions, including the Vector Mix, Cloning Buffer, Control Insert, SoloPack Competent Cells, and pUC18 Control Plasmid.
| Reactions supported | 21 reactions (1 µl each) |
|---|---|
| Control Insert quantity | 50 ng |
| Competent Cells volume per tube | 50 µl |
| Control Plasmid concentration | 0.1 ng/µl in TE buffer |
| Storage temperature for cells and plasmid | -80°C |
The SoloPack Competent Cells must be stored at -80°C, preferably at the bottom of a freezer.
Heat-shock the transformation mixture at 42°C for 45 seconds.