Key facts
- Brand
- STRATAGENE
- Model
- XL10‑Gold
- Category
- Other
- Industry
- Health
- Documents
- 2
- Languages
- ZH · EN
- Last added
- 2026-09-30
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Stratagene XL10‑Gold Chemically Competent Cell User Manual
The XL10‑Gold Chemically Competent Cell is a high‑efficiency strain developed by Stratagene for transforming large plasmids or valuable ligation products. It is supplied as 100 µl vials (CAT# DL1050) with a genotype that removes most restriction systems and endonucleases, enhancing DNA yield and stability.
The manual describes the recommended storage at –80 °C (up to 6 months), the standard heat‑shock transformation procedure (42 °C for 30 s), and media preparation. Precautions such as gentle handling of cells and antibiotic resistance limits are also included. This manual is written in Chinese.
Stratagene XL10‑Gold specifications
| Transformation efficiency | >2 × 10⁹ cfu/µg DNA |
|---|---|
| Storage condition | -80 °C for 6 months |
| Genotype | Tet R Δ(mcrA)183 Δ(mcrCB‑hsdSMR‑mrr)173 endA1 supE44 thi‑1 recA1 gyrA96 relA1 lac Hte |
| Cell amount per vial | 100 µl |
Source: Stratagene XL10‑Gold Chemically Competent Cell User Manual
All Stratagene XL10‑Gold documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
What is the recommended heat‑shock temperature and duration?
Heat‑pulse the cells in a 42 °C water bath for 30 seconds.
From: Stratagene XL10‑Gold Chemically Competent Cell User Manual
How should XL10‑Gold cells be stored?
Store the cells at –80 °C, where they remain viable for up to six months.
From: Stratagene XL10‑Gold Chemically Competent Cell User Manual
Can XL10-Gold ultracompetent cells be stored in liquid nitrogen?
No, the document explicitly states not to store the cells in liquid nitrogen.
From: Agilent XL10-Gold Ultracompetent Cells Product Certificate
What is the optimal heat-pulse duration for transformation?
The optimal duration is 30 seconds at 42°C, as efficiency decreases sharply outside this range.
From: Agilent XL10-Gold Ultracompetent Cells Product Certificate