YieldAce Hotstart DNA Polymerase INSTRUCTION MANUAL is the instruction manual of Agilent product, which introduces the features and usage of the product
This document is the instruction manual for Agilent Technologies' YieldAce Hotstart DNA Polymerase. It provides ordering information, materials provided in kits with catalog numbers 600334 (1000 U) and 600336 (3000 U), and storage conditions at -20°C.
The manual details critical optimization parameters for PCR reactions, including suggested input template amounts, enzyme concentration (2.5U per 50 ul reaction), dNTP concentrations, primer design guidelines, and cycling parameters such as denaturation at 95°C and extension at 72°C. It also includes troubleshooting advice for issues like low yield or multiple bands.
| Catalog Numbers | #600334 (1000 U) and #600336 (3000 U) |
|---|---|
| Storage Temperature | -20°C |
| Suggested Enzyme Concentration (50 ul) | 2.5U |
| Recommended dNTP Concentration | 200 uM each dNTP (0.8 mM total) |
| Recommended Primer Concentration | ~100 ng of each primer (for 50-ul reaction) |
| Denaturation Temperature | 95°C |
| Extension Temperature | 72°C |
The available catalog numbers are #600334 (1000 U) and #600336 (3000 U).
The suggested condition is to use 2.5U of YieldAce hotstart DNA polymerase per 50 ul reaction.
A denaturation temperature of 95°C is suggested, along with an extension temperature of 72°C.
All components must be stored at -20°C.