This document provides instructions for using the StrataClone Mammalian Expression Vector Systems, including the features and characteristics of different tagging systems.
The document details the StrataClone Mammalian Expression Vector Systems, which include five specific kits (catalogs #240228 through #240232) designed for creating expression plasmids. Each kit contains vector mixes, blunt cloning buffer, competent cells, and control materials to facilitate 21 reactions.
| Kit Capacity | 21 reactions (1 ul each) |
|---|---|
| Competent Cells Volume | 50 ul per transformation |
| Control Insert Concentration | 5 ng/ul |
| Storage Temperature for Cells | -80°C |
They must be stored at -80°C, specifically at the bottom of a freezer.
A proofreading DNA polymerase, thermocycler, and specific agar plates.
It serves as a control for the cloning reactions.