This document is the instruction manual for the StrataClone Blunt PCR Cloning Kit, providing information on the usage and limited product warranty of the kit.
The StrataClone Blunt PCR Cloning Kit allows for high-efficiency, 5-minute cloning of blunt-ended PCR products. The technology utilizes the DNA rejoining activity of Vaccinia virus topoisomerase I to ligate blunt-ended PCR products to vector arms in a rapid reaction without clean-up steps.
| Reaction Capacity | 20 experimental cloning reactions plus one Control Insert reaction |
|---|---|
| Vector Size | 4.3 kb |
| Cloning Time | 5 minutes |
The StrataClone SoloPack Competent Cells must be stored at –80°C.
Users must provide a proofreading DNA polymerase, thermocycler, and LB medium with antibiotics.