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- Last added
- 2026-09-30
STRATAGENE models by category
Featured STRATAGENE manuals
Stratagene 200514 QuikChange® Multi Site-Directed Mutagenesis Kit Instruction Manual
This manual describes the contents of the QuikChange® Multi Site-Directed Mutagenesis Kit, the required materials, storage conditions, and detailed three‑step mutagenesis protocol. It also includes guidelines for mutagenic primer design, transformation of XL10‑Gold® ultracompetent cells, and troubleshooting tips.
The kit supplies enzyme blend, reaction buffer, dNTP mix, Dpn I enzyme, control template and primers, and XL10‑Gold® cells, enabling rapid generation of multiple site‑directed mutations in a single reaction.
Covers: Materials Provided · Storage Conditions · Mutagenic Primer Design · Three‑Step Mutagenesis Protocol · Transformation of XL10‑Gold® Cells · Troubleshooting
Stratagene XL10‑Gold Chemically Competent Cell User Manual
The XL10‑Gold Chemically Competent Cell is a high‑efficiency strain developed by Stratagene for transforming large plasmids or valuable ligation products. It is supplied as 100 µl vials (CAT# DL1050) with a genotype that removes most restriction systems and endonucleases, enhancing DNA yield and stability.
The manual describes the recommended storage at –80 °C (up to 6 months), the standard heat‑shock transformation procedure (42 °C for 30 s), and media preparation. Precautions such as gentle handling of cells and antibiotic resistance limits are also included. This manual is written in Chinese.
Covers: Product Specifications · Product Description · Standard Operating Procedure · Stratagene Standard Protocol · Media and Reagents Preparation · Precautions
Stratagene QPCR Human Reference Total RNA Instruction Manual
The QPCR Human Reference Total RNA is a high‑quality control material composed of total RNA from ten human cell lines, designed for quantitative PCR gene‑expression analysis. It is supplied as 25 µg of RNA and stored at –80 °C to maintain integrity.
The manual details the materials provided, required equipment, storage conditions, assay‑optimization protocols for SYBR® Green, TaqMan® and molecular beacon assays, standard‑curve generation, and troubleshooting tips. It also includes technical specifications, recommended primer and probe concentrations, and instrument settings for Stratagene’s Mx series and ABI PRISM platforms.
Covers: Materials Provided · Storage Conditions · Assay Optimization Applications · QRT‑PCR Assay Optimization Protocol · Troubleshooting
Stratagene Mx3000P and Mx3005P QPCR Systems Product Leaflet
The Mx3000P and Mx3005P are 96‑well, real‑time quantitative PCR instruments with four and five user‑selectable optical channels respectively. Both systems use a single photomultiplier tube for high sensitivity and a Peltier‑based thermal design that ensures uniform heating across wells.
The instruments are shipped with the MxPro QPCR Software, which offers easy assay setup, real‑time data viewing, and multiple export formats. A full line of Brilliant II QPCR reagents and master mixes is listed for use with these platforms, providing high sensitivity and a wide dynamic range.
Covers: Features of the Mx3000P QPCR System · Features of the Mx3005P QPCR System · Advanced Optical System · Mx3005P Optical Filters · Powerful Data Analysis Software · MxPro QPCR Software Features
Latest STRATAGENE manuals
Stratagene 200514 QuikChange® Multi Site-Directed Mutagenesis Kit Instruction Manual
User Manual 24 pages · 292 KB · ENThe Stratagene QuikChange® Multi Site-Directed Mutagenesis Kit (catalog #200514) instruction manual provides step‑by‑step protocols, primer design…
Stratagene XL10‑Gold Chemically Competent Cell User Manual
User Manual 2 pages · 2.2 MB · ZHThe Stratagene XL10‑Gold Chemically Competent Cell manual provides specifications, storage conditions, and detailed transformation protocols.
Stratagene QPCR Human Reference Total RNA Instruction Manual
User Manual 18 pages · 142 KB · ENThe Stratagene QPCR Human Reference Total RNA instruction manual provides usage, storage, assay optimization, standardization, and troubleshooting guidance.
Stratagene Mx3000P and Mx3005P QPCR Systems Product Leaflet
Product Leaflet 18 pages · 3.4 MB · ENThe Stratagene product leaflet provides an overview of the Mx3000P and Mx3005P real‑time QPCR instruments, their optical features, software, and compatible…
STRATAGENE Brilliant II QPCR QRT-PCR Reagents Manual
Our next generation Brilliant II® QPCR and QRT-PCR Master Mix Kits offer superior sensitivity for improved quantification and reproducibility. Our new Brilliant® II QPCR and QRT-PCR reagents were developed to maintain the high quality of our original Brilliant product line, but with a significant improvement in sensitivity of detection, ensuring reproducible quantification even at low target concentrations.
STRATAGENE DNA Markers Manual
DNA Marker product introduction
STRATAGENE Alien QRT-PCR Inhibitor Alert Manual
Alien® QRT-PCR Inhibitor Alert is a high-quality external control for detecting inhibitors in RNA samples for QRT-PCR experiments.
STRATAGENE Brilliant II FAST Brilliant II FAST SYBR QPCR Kits Manual
The Brilliant II and Brilliant II FAST SYBR QPCR Master Mixes are single-tube reagents designed for performing quantitative PCR amplifications with an accelerated cycling protocol that completes 40 cycles of PCR in approximately 48 minutes. The master mix formulations and fast cycling protocol were developed together to create a system for completing QPCR reactions in less time without compromising target detection sensitivity, specificity, or reproducibility. The Brilliant II QPCR Master Mixes have been successfully used with fluorescent TaqMan probes and SYBR Green to amplify and detect a variety of DNA targets, including genomic DNA, plasmid DNA, and cDNA. The Brilliant II FAST QPCR kit includes 2× master mix containing hot start Taq DNA polymerase, dNTPs, Mg2+, and a buffer specially formulated for fast cycling. The Brilliant II FAST SYBR QPCR Master Mix also includes the DNA binding dye SYBR Green for detection. The DNA polymerase features a rapid hot start capability that reduces nonspecific product formation while keeping the run time of the PCR protocol to a minimum, making it ideal for the Brilliant II QPCR kits. Both Brilliant II FAST master mixes have been developed, tested, and validated on our Mx3000P and Mx3005P QPCR systems. By utilizing our Mx QPCR systems in combination with our new Brilliant II FAST QPCR kits
STRATAGENE Easy-A High Fidelity PCR Cloning Enzyme Manual
STRATAGENE New protocols for Isolating High Molecular-Weight Genomic DNA Manual
STRATAGENE AccuScript High Fidelity Reverse Transcriptase Products Manual
AccuScript™ High Fidelity Reverse Transcriptase products are the best reverse transcriptase for first-strand cDNA synthesis, producing the highest transcription accuracy possible. It is the ideal choice for any application that requires premium error-free cDNA.
STRATAGENE AffinityScriptTM Multiple Temperature Reverse Transcriptase Manual
AffinityScript ™ Multiple Temperature Reverse Transcriptase is an engineered enzyme with high affinity for primer template complexes and can synthesize cDNA across a broad range of temperatures from 37°C to 55°C. The enzyme promotes cDNA yield due to its high affinity for primer:template complexes.
STRATAGENE SideStep II Lysis Stabilization Products Manual
SideStep ™ II QRT-PCR products allow you to skip RNA purification and go directly to QRT-PCR
STRATAGENE SpotReport Alien Array Validation System is a group of controls that collectively validates microarray data Manual
The SpotReport® Alien® Array Validation System* is a group of controls that collectively validates microarray data. They can be used for any genome microarray and play a pivotal role in normalization, monitoring hybridization specificity, and checking overall data quality from each microarray.
STRATAGENE MycoSensor PCR Assay Kit for Fast Mycoplasma Detection Manual
This document describes a rapid method for detecting bacteria using PCR. The method can detect eight of the most common species of bacteria in as little as three hours and can avoid false positive and false negative results.
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in STRATAGENE manuals
What is the catalog number of the QuikChange® Multi Site-Directed Mutagenesis Kit?
The kit is catalog #200514.
From: Stratagene 200514 QuikChange® Multi Site-Directed Mutagenesis Kit Instruction Manual
At what temperature should the kit components be stored?
XL10‑Gold® ultracompetent cells and the control plasmid are stored at –80 °C, while all other components are stored at –20 °C.
From: Stratagene 200514 QuikChange® Multi Site-Directed Mutagenesis Kit Instruction Manual
What is the recommended heat‑shock temperature and duration?
Heat‑pulse the cells in a 42 °C water bath for 30 seconds.
From: Stratagene XL10‑Gold Chemically Competent Cell User Manual
How should XL10‑Gold cells be stored?
Store the cells at –80 °C, where they remain viable for up to six months.
From: Stratagene XL10‑Gold Chemically Competent Cell User Manual
What is the recommended storage condition for the QPCR Human Reference Total RNA?
Store all components at –80 °C.
From: Stratagene QPCR Human Reference Total RNA Instruction Manual
How much reference RNA is provided in the kit?
The kit supplies 25 µg of RNA (1 µg/µl in 0.1 mM EDTA/RNase‑free water).
From: Stratagene QPCR Human Reference Total RNA Instruction Manual