This manual introduces the use of PCR-Script Amp Electroporation-Competent Cell Cloning Kit
This document is an instruction manual for the Agilent PCR-Script Amp Electroporation-Competent Cell Cloning Kit (Catalog #211186). It provides comprehensive details on the materials provided within the kit, including specific reagents like cloned Pfu DNA polymerase and electroporation-competent cells, along with their storage conditions. The manual outlines the necessary additional equipment required for the procedure.
| XL1-Blue MRF´ Kan electroporation-competent cells quantity | 13 × 80 μl |
|---|---|
| White colonies percentage with Pfu DNA polymerase | ≥80% |
The dNTP mix should be stored at –80°C for long-term storage, though it may be kept at –20°C for up to 3 months.
They must be stored immediately at –80°C and transferred from the bottom of a freezer to avoid efficiency loss.
The capacity of each microspin cup is approximately 0.8 ml.