This manual introduces the use of PowerPlex® Matrix Standard 3100/3130 products, including product composition, storage conditions, instrument preparation and use of version 2.0 or data collection software for spectral calibration.
This manual describes how to prepare the PowerPlex® Matrix Standard reagents, including Fluorescein, JOE, TMR, and CXR matrices, and how to load them onto ABI PRISM® 3100, 3100‑Avant, 3130, and 3130xl instruments. It also covers instrument configuration, protocol management, spectral calibration, and data analysis steps required for STR typing.
Additional sections address licensing information for PCR patents, troubleshooting tips, and contact details for technical support. The document includes tables of reaction volumes, RFU performance ranges, and kit capacities for various PowerPlex® systems. This manual is written in Chinese.
| Fluorescein Matrix volume | 25 µl |
|---|---|
| TMR Matrix volume | 25 µl |
| CXR Matrix volume | 25 µl |
| RFU signal range | 1,000–4,000 RFU |
| Reactions per kit (DC6531 PowerPlex® 16 System) | 100 reactions |
The kit works with ABI PRISM® 3100, 3100‑Avant, 3130, and 3130xl instruments.
Matrix signals should fall between 1,000 and 4,000 RFU.
Contact Hoffmann‑La Roche for a PCR license.