This manual describes the CytoTox 96® non‑radioactive cytotoxicity assay, which quantifies lactate dehydrogenase (LDH) released from lysed cells using a colorimetric tetrazolium reaction. It outlines the assay principle, required reagents, and the calculation of cytotoxicity percentages.
The document includes detailed protocols for setting up 96‑well plates, preparing controls, optimizing target cell numbers, and performing single‑cell population measurements. It also provides storage conditions for reagents, background‑absorbance correction methods, and a troubleshooting guide for common issues such as high background or low signal. This manual is written in Chinese.
| Assays per kit | 1,000 (G1780) |
|---|---|
| Storage temperature for Substrate Mix and Assay Buffer | -20 °C |
| Storage temperature for LDH Positive Control, Lysis Solution (10X) and Stop Solution | 4 °C |
The kit provides 1,000 assay reactions (G1780).
Store the substrate mix at –20 °C.
Measure absorbance at 490 nm (or 492 nm).