A technical guide from Promega describing 3D cell culture assays for viability, apoptosis, metabolism and oxidative stress detection.
This guide introduces 3D cell culture technology and its advantages over traditional 2D methods. It explains the definition, applications, and classification of 3D cultures, and outlines how they better mimic in‑vivo environments for disease modeling and drug discovery.
The document details Promega’s assay portfolio for 3D cell health analysis, including ATP‑based CellTiter‑Glo® 3D viability, RealTime‑Glo™ MT viability, Caspase‑Glo® 3/7 apoptosis, ROS‑Glo™ H2O2 oxidative stress, and GSH‑Glo™ glutathione assays. It provides assay principles, protocols, catalog numbers, and compatible detection instruments such as GloMax® readers. This manual is written in Chinese.
| CellTiter‑Glo® 3D Viability Assay catalog number (100 assays) | G9681 |
|---|---|
| Caspase‑Glo® 3/7 3D Assay catalog number (100 assays) | G8981 |
| ROS‑Glo™ H2O2 Assay catalog number (100 assays) | G8820 |
Incubate for 30 minutes at room temperature before reading the luminescence.
Live cells reduce a substrate to produce NanoLuc® luciferase, which emits light proportional to viable cell number.
The assay is listed under catalog numbers G8981 (100 assays), G8982 (1,000 assays), and G8983 (10×100 assays).