The Promega LS1040 Eastep Super Total RNA Extraction Kit manual provides step‑by‑step instructions, reagent preparation, storage guidelines and…
This technical manual describes the Eastep® Super Total RNA Extraction Kit (LS1040) from Promega. It outlines the kit components, storage conditions, reagent preparation, and detailed protocols for extracting total RNA from animal tissues, cultured cells, plant material and bacteria.
The document also includes guidelines to prevent RNase contamination, recommended sample starting amounts, a complete purification workflow, yield data for different sample types, and a troubleshooting section with solutions to common problems such as low A260/A230 ratios or RNA degradation. This manual is written in Chinese.
| Number of extractions per kit | 50 |
|---|---|
| RNA lysis buffer (SR220A) | 40 ml |
| RNA dilution buffer (SR221A) | 30 ml |
| RNA wash buffer (SR222A) | 40 ml |
| 10X DNAse I buffer (SR223A) | 0.3 ml |
| 1‑Thioglycerol (SR224A) | 1 ml |
One LS1040 kit provides enough reagents for 50 total RNA extractions from tissues, cells or bacteria.
The 10X DNAse I buffer and 1‑thioglycerol should be stored at 4 °C; other components at 15 °C–30 °C; the prepared DNAse I solution at –20 °C; RNA lysis buffer with 1‑thioglycerol at +4 °C for up to 6 months.
Add NaCl to a final concentration of 0.1 M, then add 2.5 × volume of anhydrous ethanol, incubate at –20 °C for 30 min, centrifuge at 10 000 g, and resuspend the RNA in RNase‑free water.