StemElite™ ID System is a kit for cell line identification and verification based on short tandem repeat (STR) markers
The StemElite ID System G9530 is a multiplex STR profiling kit for cell line authentication and contamination detection. It combines a master mix, primers, internal standards and allelic ladders to enable simultaneous amplification of ten human loci and one mouse locus.
The manual provides detailed information on product components, storage conditions, required PCR and capillary electrophoresis instruments, step‑by‑step DNA preparation, PCR cycling, matrix calibration, electrophoresis setup, data analysis with GeneMapper software, and a troubleshooting guide with common issues and recommended solutions. This manual is written in Chinese.
| Number of reactions per kit | 50 |
|---|---|
| Reaction volume per assay | 25 µL |
| Storage temperature | -20 °C |
| Light sensitivity | Avoid light exposure |
Each StemElite ID reaction should use 2 ng of DNA template.
Promega recommends 10/22 cycles (10 cycles at the first temperature, 22 cycles at the second) for 2 ng of template DNA.
Supported instruments include Applied Biosystems 3130 or 3130 xl, ABI PRISM® 3100, and ABI PRISM® 3100‑Avant Genetic Analyzers.
All reagents should be stored at –20 °C in a non‑defrosting freezer and protected from light.