This document describes Promega's Wizard® Plus SV Minipreps DNA Purification System, which provides a simple and reliable method for rapid isolation of plasmid DNA. It is suitable for purifying plasmid DNA from E. Coli and can be used for DNA fluorescent sequencing and other molecular biology applications.
The Wizard Plus SV Miniprep DNA Purification System provides a fast, reliable method for isolating plasmid DNA from E. coli. The kit supplies enough reagents for 10, 50 or 250 preparations, each handling 1‑10 ml of overnight culture.
The manual details product components, step‑by‑step operation (cell suspension, lysis, neutralization, column washing, DNA elution), buffer preparation, storage conditions, and troubleshooting tips for low yield or DNA damage. It also lists related products and references for further reading. This manual is written in Chinese.
| Processing volume per sample | 1‑10 ml |
|---|---|
| Storage temperature | 22‑25 °C |
| Column wash solution composition | 168.2 mM Tris‑HCl (pH 7.5), 22.6 mM acetate‑K, 0.109 mM EDTA |
| DNA elution stability | Stable at –20 °C (no buffer) or in TE at 4 °C |
Each preparation can handle up to 10 ml of overnight E. coli culture.
Add 35 ml of 95 % ethanol to the wash solution to reach a final volume of 55 ml.
No, components from Wizard Plus and Wizard Plus SV kits are not interchangeable.
Store all reagents at 22‑25 °C.