This manual introduces the use of NGF Emax ®immunoassay system, which uses antibody sandwich method to sensitively and specifically measure the content of NGF. It is suitable for the quantitative determination of NGF in tissue culture supernatant or tissue extracts, with a quantitative range of 7.8-500 pg/ml.
This manual provides step‑by‑step instructions for performing the NGF ELISA assay using Promega's kit. It covers reagent preparation, plate coating, sample handling, and detection using TMB substrate.
Safety precautions, recommended storage conditions, and detailed specifications of the kit components are also included to ensure reliable results. This manual is written in Chinese.
| Detection range | 7.8‑500 pg/ml |
|---|---|
| Included buffer | 5X Buffer (5X封団和桂品液) |
| Capture antibody | Anti‑NGF pAb |
| Detection antibody | Anti‑NGF mAb |
| Secondary antibody | HRP Conjugate (HRP‑IgG) |
| Substrate | TMB One Solution |
The kit can quantify NGF from 7.8 pg/ml up to 500 pg/ml.