This manual describes the use of the Tissue and Hair Extraction Kit (for use with the DNA IQTM System). The kit is intended to extract and purify double-stranded or single-stranded genomic DNA and mitochondrial DNA from a variety of tissues and hair.
This manual introduces the Promega Tissue and Hair Extraction Kit (model DC6740) designed for use with the DNA IQ™ system. It describes the kit components, including incubation buffer, proteinase K, and DTT, and explains how they enable efficient lysis of a wide range of sample types such as hair follicles, fresh or frozen tissue, formalin‑fixed and paraffin‑embedded specimens.
Detailed protocols cover reagent preparation, incubation steps at 56 °C, addition of lysis buffer and magnetic resin, multiple wash cycles, and DNA elution. The document also includes a troubleshooting section, reference list, and a list of related Promega products. This manual is written in Chinese.
| Number of reactions per kit | 100 |
|---|---|
| Incubation Buffer volume | 50 ml |
| Proteinase K amount | 100 mg |
| Proteinase K final concentration in solution | 1.8 mg/ml |
The protocol calls for incubation at 56 °C for the specified time.
Three wash cycles are performed, each repeated twice as described in the procedure.