Chinese operation manual for Promega TB281 luciferase detection system, covering components, preparation, assay methods and safety notes.
This manual introduces the Promega TB281 Luciferase Detection System, describing its purpose for reporter gene assays and the advantages of firefly luciferase. It lists the system’s product variants (E1483, E1500, E1501, E4030, E4530, E4550) and provides detailed information on reagents, buffers, and recommended applications for mammalian, plant and bacterial samples.
The document explains how to prepare reagents, perform assays with manual or auto‑injector luminometers, and includes general precautions such as temperature equilibration, storage conditions, and instrument settings. Additional sections cover buffer compositions, related products, and references for further reading. This manual is written in Chinese.
| Detection limit (luminescence meter) | 10‑20 mol (0.001 pg) luciferase |
|---|---|
| Measurement time | 10 seconds |
| Signal stability | ≥ 60 seconds |
| Typical delay time | 2 seconds |
| Reagent storage (‑20 °C) | stable for 1 month |
| Reagent storage (‑70 °C) | stable for 1 year |
Each kit contains enough reagents for either 100 or 1,000 standard detections depending on the product variant (e.g., E1500 – 100 detections, E1501 – 1,000 detections).
Reagents should be brought to room temperature (≈22‑25 °C) and allowed to equilibrate for at least 30 minutes before the assay.
Set a delay of 2 seconds and a measurement time of 10 seconds for typical assays.
Store the reagent at –20 °C for up to one month or at –70 °C for up to one year, avoiding repeated freeze‑thaw cycles.