Key facts
- Manuals
- 15
- Models
- 1
- Categories
- 1
- Languages
- ZH
- Last added
- 2026-10-01
Promega models by category
Featured Promega manuals
Promega TB281 Luciferase Detection System User Manual
This manual introduces the Promega TB281 Luciferase Detection System, describing its purpose for reporter gene assays and the advantages of firefly luciferase. It lists the system’s product variants (E1483, E1500, E1501, E4030, E4530, E4550) and provides detailed information on reagents, buffers, and recommended applications for mammalian, plant and bacterial samples.
The document explains how to prepare reagents, perform assays with manual or auto‑injector luminometers, and includes general precautions such as temperature equilibration, storage conditions, and instrument settings. Additional sections cover buffer compositions, related products, and references for further reading. This manual is written in Chinese.
Covers: Introduction · Product Components · Preparation for Luciferase Assay · Luciferase Assay Methods · General Precautions · Solutions and Buffers Composition
Promega 3D Cell Analysis Product Guide
This guide introduces 3D cell culture technology and its advantages over traditional 2D methods. It explains the definition, applications, and classification of 3D cultures, and outlines how they better mimic in‑vivo environments for disease modeling and drug discovery.
The document details Promega’s assay portfolio for 3D cell health analysis, including ATP‑based CellTiter‑Glo® 3D viability, RealTime‑Glo™ MT viability, Caspase‑Glo® 3/7 apoptosis, ROS‑Glo™ H2O2 oxidative stress, and GSH‑Glo™ glutathione assays. It provides assay principles, protocols, catalog numbers, and compatible detection instruments such as GloMax® readers. This manual is written in Chinese.
Covers: 什么是 3D细胞培养 · 3D细胞培养的应用 · 3D细胞培养的分类 · 3D培养细胞的检测 · 检测仪器 · 细胞活力检测
Promega LS1040 Eastep Super Total RNA Extraction Kit User Manual
This technical manual describes the Eastep® Super Total RNA Extraction Kit (LS1040) from Promega. It outlines the kit components, storage conditions, reagent preparation, and detailed protocols for extracting total RNA from animal tissues, cultured cells, plant material and bacteria.
The document also includes guidelines to prevent RNase contamination, recommended sample starting amounts, a complete purification workflow, yield data for different sample types, and a troubleshooting section with solutions to common problems such as low A260/A230 ratios or RNA degradation. This manual is written in Chinese.
Covers: Product description · Kit components · Storage conditions · Reagent preparation · RNase contamination prevention · Cell disruption methods
Promega DC6740 Tissue and Hair Extraction Kit Operation Manual
This manual introduces the Promega Tissue and Hair Extraction Kit (model DC6740) designed for use with the DNA IQ™ system. It describes the kit components, including incubation buffer, proteinase K, and DTT, and explains how they enable efficient lysis of a wide range of sample types such as hair follicles, fresh or frozen tissue, formalin‑fixed and paraffin‑embedded specimens.
Detailed protocols cover reagent preparation, incubation steps at 56 °C, addition of lysis buffer and magnetic resin, multiple wash cycles, and DNA elution. The document also includes a troubleshooting section, reference list, and a list of related Promega products. This manual is written in Chinese.
Covers: Introduction · Product Components · Operation Steps · Reagent Preparation · DNA Purification from Hair Follicles · DNA Purification from Tissue Samples
Latest Promega manuals
Promega TB281 Luciferase Detection System User Manual
User Manual 14 pages · 296 KB · ZHChinese operation manual for Promega TB281 luciferase detection system, covering components, preparation, assay methods and safety notes.
Promega 3D Cell Analysis Product Guide
Product Leaflet 20 pages · 6.3 MB · ZHA technical guide from Promega describing 3D cell culture assays for viability, apoptosis, metabolism and oxidative stress detection.
Promega LS1040 Eastep Super Total RNA Extraction Kit User Manual
User Manual 16 pages · 725 KB · ZHThe Promega LS1040 Eastep Super Total RNA Extraction Kit manual provides step‑by‑step instructions, reagent preparation, storage guidelines and…
Promega DC6740 Tissue and Hair Extraction Kit Operation Manual
User Manual 7 pages · 384 KB · ZHThe Promega DC6740 Tissue and Hair Extraction Kit operation manual provides step‑by‑step procedures, reagent preparation, and troubleshooting for DNA…
Promega Wizard Plus SV Miniprep DNA Purification System User Manual
User Manual 11 pages · 290 KB · ZHChinese user manual for Promega Wizard Plus SV miniprep kit, describing components, procedures, troubleshooting and buffer recipes.
Promega PowerPlex Matrix Standard3100/3130 DG4650 service manual
User Manual 15 pages · 88 KB · ZHThe Promega PowerPlex Matrix Standard 3100/3130 Service Manual provides detailed instructions for reagent preparation, instrument setup, and protocol…
Promega CytoTox 96 G1780 Non‑Radioactive Cytotoxicity Assay User Manual
User Manual 13 pages · ZHThe Promega CytoTox 96 G1780 kit manual provides step‑by‑step instructions, component details and troubleshooting for the non‑radioactive LDH cytotoxicity…
Promega Z3351 Total RNA mini-Isolation System User Manual
User Manual 15 pages · 346 KB · ZHThe Promega Z3351 Total RNA mini‑Isolation System User Manual provides step‑by‑step instructions for manual and automated purification of total RNA from…
Promega StemElite ID System G9530 Service Manual
Service Manual 32 pages · 2 MB · ZHService manual for Promega StemElite ID System G9530 covering components, storage, instrument requirements, DNA preparation, PCR setup, electrophoresis…
PROMEGA PowerPlex Y system products DC6760AndDC676 Manual
User Manual 41 pages · 886 KB · ZHThis Promega user manual describes the PowerPlex Y System kits DC6760 and DC6761, covering reagents, protocols, safety, and data analysis.
Promega A1360/A1380/A3600/A3610 pGEM®-T and pGEM®-T Easy Vector System User Manual
User Manual 23 pages · 465 KB · ZHOperating instructions for Promega pGEM®-T and pGEM®-T Easy cloning vectors (models A1360, A1380, A3600, A3610).
Promega DC6700/DC6701 DNA IQTM System Small Sample Case Manual
User Manual 8 pages · 529 KB · ZHChinese operation manual for Promega DNA IQTM System (DC6700/DC6701) covering small‑sample DNA extraction and troubleshooting.
Promega NGF ELISA Kit User Manual
User Manual 11 pages · 259 KB · ZHUser manual for Promega NGF ELISA kit (models G7630 and G7631) describing reagents, plate preparation, and assay procedure.
Promega Matrix FL-JOE-TMR-CXR Service Manual
Service Manual 12 pages · 347 KB · ZHService manual for Promega Matrix FL-JOE-TMR-CXR providing preparation, operation, troubleshooting and buffer recipes.
promega DC6760 DC676 PowerPlex Y system Manual
Which document do I need?
User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Service Manual
Disassembly, diagnostics and part numbers, for repair.
Questions answered in Promega manuals
How many detections does each TB281 system kit provide?
Each kit contains enough reagents for either 100 or 1,000 standard detections depending on the product variant (e.g., E1500 – 100 detections, E1501 – 1,000 detections).
At what temperature should the luciferase assay reagents be equilibrated before use?
Reagents should be brought to room temperature (≈22‑25 °C) and allowed to equilibrate for at least 30 minutes before the assay.
How long should the CellTiter‑Glo® 3D assay be incubated at room temperature?
Incubate for 30 minutes at room temperature before reading the luminescence.
What is the detection principle of the RealTime‑Glo™ MT Cell Viability Assay?
Live cells reduce a substrate to produce NanoLuc® luciferase, which emits light proportional to viable cell number.
How many RNA extractions can be performed with one LS1040 kit?
One LS1040 kit provides enough reagents for 50 total RNA extractions from tissues, cells or bacteria.
From: Promega LS1040 Eastep Super Total RNA Extraction Kit User Manual
What are the recommended storage conditions for the kit reagents?
The 10X DNAse I buffer and 1‑thioglycerol should be stored at 4 °C; other components at 15 °C–30 °C; the prepared DNAse I solution at –20 °C; RNA lysis buffer with 1‑thioglycerol at +4 °C for up to 6 months.
From: Promega LS1040 Eastep Super Total RNA Extraction Kit User Manual