Matrix FL-JOE-TMR-CXR is a standard sample for evaluating multi-color systems with ABI PRISM® 310 Genetic Analyzer and ABI PRISM® 377 DNA Sequencer. This product contains DNA fragments labeled with 4 kinds of fluorescent dyes, which are used to generate blue, green, yellow and red standards respectively. A Matrix is required for each instrument.
This manual describes how to prepare, run and troubleshoot the Matrix FL-JOE‑TMR‑CXR kit used with ABI PRISM genetic analysis instruments. It includes step‑by‑step instructions for instrument setup, sample preparation, capillary electrophoresis, matrix generation, and optimization.
The document also lists safety warnings for reagents, detailed buffer compositions, storage conditions, and a troubleshooting guide for common issues such as baseline spikes and peak inversion. All procedures are intended for use with Promega's PowerPlex STR systems. This manual is written in Chinese.
| Matrix composition | Fluorescein, JOE, TMR, CXR labeled DNA fragments |
|---|---|
| Storage temperature | -20°C |
| Formamide conductivity requirement | <100 µS/cm |
| Minimum peaks for matrix generation | At least 5 peaks |
All matrix components should be stored at –20 °C.
The formamide must have a conductivity of less than 100 µS/cm.
A matrix requires at least five distinct peaks in the electropherogram.
It is designed for use with PowerPlex 16 and other Promega fluorescent STR systems on ABI PRISM instruments.