The GeneCopoeia miProfile miRNA qPCR Primer (catalog HmiRQP90 21) manual provides usage instructions, validation data, and recommended reagents for…
The miProfile miRNA qPCR Primer is a set of upstream primers designed for specific detection of mature miRNA by qPCR. It is intended to be used together with GeneCopoeia's All‑in‑One miRNA qRT‑PCR Detection Kit for research‑only quantitative analysis.
The document includes product information, recommended reagents, detailed usage procedures (centrifugation, dilution to 2 µM, final 0.2 µM in the reaction), application notes, and a validation report showing experimental setup on a Bio‑Rad iQ5 Real‑Time PCR system with RNA extraction, reverse transcription, and qPCR results. This manual is written in Chinese.
| Catalog number | HmiRQP90 21 |
|---|---|
| Primer ID | hsnoRNA U48 |
| Package size | 200 rxn |
It should be used with GeneCopoeia All‑in‑One miRNA qRT‑PCR Detection Kit (Catalog Nos. AOMD‑Q020 or AOMD‑Q050).
Validation was performed on a Bio‑Rad iQ5 Real‑Time PCR Detection System.
Store the primer at –20 °C and avoid repeated freeze‑thaw cycles.