The document describes the features and characteristics of the E.coli Competent Cells BLR(DE3)plysS product kit, including the characteristics of BL21-Gold{DE3} competent cells, high transformation efficiency, and high protein expression yield.
The U0109A manual provides an overview of GeneCopoeia's BLR(DE3) plysS competent cells, highlighting their high transformation efficiency (up to 1×10⁸ cfu/µg DNA) and suitability for high‑yield plasmid preparation. It also lists the recommended storage conditions and the composition of the control DNA (pUC19).
Step‑by‑step transformation instructions cover thawing, mixing DNA (≤10 ng), heat‑shock at 42 °C, recovery in SOC or LB medium, and incubation conditions. Safety precautions emphasize maintaining cells at –80 °C, avoiding repeated freeze‑thaw cycles, and using low‑retention tubes for best results. This manual is written in Japanese.
| Cell Strain | BLR(DE3) plysS |
|---|---|
| Transformation Efficiency | 1×10⁸ cfu/µg DNA |
| Control DNA | pUC19 (10 pg/µl) |
Heat‑shock at 42 °C for 30–60 seconds.
Yes, but using LB may reduce the transformation efficiency.