This document introduces how to reduce HPLC solvent consumption by using short LC columns with small internal diameters and particles to combat the worldwide shortage of acetonitrile.
This document addresses the worldwide shortage of acetonitrile by detailing strategies to reduce its consumption in High-Performance Liquid Chromatography (HPLC). It outlines methods such as using columns with smaller internal diameters, shorter lengths, and sub-2-micron particles to achieve significant cost and solvent savings.
| Acetonitrile price (Jan 2009) | 100 US$ per liter |
|---|---|
| Solvent reduction (smaller ID) | 50 to 60 % |
| Solvent reduction (shorter length) | up to 70 % |
| Solvent reduction (sub-2-micron) | up to 85% |
Up to 85% reduction in solvent consumption and costs can be achieved.
Length can vary by +70%, internal diameter by +25%, and particle size can be 50% smaller but not larger.