This document describes a method to measure the accuracy and precision of the Bravo in conjunction with a 96-Channel ST Disposable Tip Head. Measurements are determined by dispensing a tartrazine/DMSO solution into dry microplates, filling the plates with water, and measuring tartrazine absorbance. The product’s performance meets or exceeds CVs of 5% and accuracy of ±10% of the desired volume across the practical volume range.
The Agilent Bravo Automated Liquid Handling Platform is designed to automate routine laboratory processes, increasing sample processing throughput and improving accuracy and precision. The platform features nine deck positions that can be configured with heating, cooling, and shaking stations, and accommodates 96-, 384-, or 1536-well SBS standard microplates.
This specific document details a technical overview of the accuracy and precision testing performed on the Bravo with a 96-Channel ST Disposable Tip Head and 30 uL tips. The test measured performance at low volumes (0.5-5 pL) using a tartrazine/DMSO solution and a UV/Vis Spectrophotometer. Results indicate that the system achieves a Coefficient of Variance (CV) of 0.9% and accuracy of 1.1% at 5 pL, with performance at volumes greater than 5 pL meeting or exceeding these metrics.
| Tip Volume Tested | 0.5-5 pL |
|---|---|
| Accuracy at 1.0 pL | 2.0% |
| Accuracy at 0.5 pL | 4.8% |
| Transfer Time at 5 pL | 37 seconds/plate |
| Transfer Time at 1.0 pL | 22 seconds/plate |
| Transfer Time at 0.5 pL | 22 seconds/plate |
At 1.0 pL, the system achieves a precision of 1.9% CV and an accuracy of 2.0%.
At 0.5 pL, the system achieves a precision of 2.9% CV and an accuracy of 4.8%.
Yes, performance at volumes >5 pL meets or exceeds the performance at 5 pL.