This document describes the solid phase extraction method for anabolic steroids using the AccuBOND II ODS (C18) 6 mL/500 mg cartridge. The sample is processed by adding methanol and water, followed by adsorption, elution, and concentration using vacuum. The residue is dissolved and injected into a gas chromatograph for analysis.
This document provides a Solid Phase Extraction (SPE) method for analyzing anabolic steroids in urine and serum samples. The procedure outlines the conditioning, loading, rinsing, and elution steps using AccuBOND ODS (C18) cartridges, along with specific equipment and reagent requirements.
The document also includes a list of target steroid compounds with their partition ratios and recovery percentages, as well as the specific Gas Chromatography (GC) conditions used for the analysis, including column specifications, temperature programming, and detector settings.
| Cartridge Type | AccuBOND ODS (C18) 6 mL/ 500 mg |
|---|---|
| Column Type | DB-1 |
| Column Dimensions | 30 m x 0.25 mm I.D. |
| Film Thickness | 0.1 pm |
| Carrier Gas | Helium at 40 cm/ sec |
| Injector Temperature | 275°C |
| Detector Type | FID |
| Detector Temperature | 300°C |
The method requires HPLC grade water and HPLC grade methanol.
The cartridge should be centrifuged at 1000-1500 rpm for 5 minutes.
The residue should be concentrated to dryness using gentle heat less than 45°C.