The document highlights the advantages of using ZORBAX SB-C18 columns for gradient separations of complex environmental mixtures, demonstrating resolution >1.5 and good peak shapes for all components. The conditions used by Robert Ricker in the environmental analysis application are ZORBAX SB-C18 column, 20% ACN/80% 0.01 M H3PO4 solution, gradient elution from 20% ACN to 45% ACN in 7.5 minutes, followed by gradient elution from 45% ACN to 80% ACN in 2.0 minutes, flow rate of 1.5 mL/min, and temperature of 35°C.
This document presents the detector response for a mixture of substituted phenols, including environmental samples. It demonstrates the chromatographic performance of Agilent ZORBAX SB-C18 columns in separating these complex mixtures.
The application highlights that gradient separations are well-suited for analyzing complex environmental mixtures using this column technology.
The ZORBAX SB-C18 column, with part number 883975-902, is used for these separations.
The flow rate is 1.5 mL/min and the temperature is set to 35°C.
All components have a resolution greater than 1.5 with good peak shapes.