This is an application note on the use of tetracycline on the Agilent ZORBAX Rx-C8 column, including peak shape and resolution.
This document describes an application for separating tetracycline antibiotics, which occur as natural products from Streptomyces bacteria or as semisynthetic lab products. Due to their high activity against both gram-positive and gram-negative bacteria, they are commonly used in pharmaceuticals and animal feed additives.
The note details a chromatographic method using a ZORBAX Rx-C8 column with a mobile phase of 75:25 0.1% TFA : Acetonitrile at low pH. This approach maintains excellent peak shape and resolution for Uracil, Minocycline, Tetracycline, Chlortetracycline, and Doxycycline.
The note demonstrates the analysis of Uracil, Minocycline, Tetracycline, Chlortetracycline, and Doxycycline.
The procedure uses a ZORBAX Rx-C8 column with dimensions of 4.6 x 150 mm.
The mobile phase is a 75:25 mixture of 0.1% TFA and Acetonitrile, flowing at 1.0 mL/min.