This document describes how new technologies can achieve excellent peak shape in the separation of tricyclic antidepressants using the Agilent XDB-C8 column and a mobile phase containing THF.
This document describes an HPLC application for separating tricyclic antidepressants (TCAs). It highlights that older column technology often resulted in broad and tailing peaks at neutral pH, but new eXtra Dense Bonding (XDB) technology allows for improved separation.
The specific method utilizes a ZORBAX Eclipse XDB-C8 column with a mobile phase containing tetrahydrofuran (THF). The text notes that THF provided the best peak shape compared to other organic solvents, enabling effective separation of compounds like Propranolol, Nortriptyline, Doxepin, and Amitriptyline at pH 7.
The separation uses a mobile phase consisting of 38% THF and 62% 25mM Potassium Phosphate at pH 7.
The column has dimensions of 4.6 x 150 mm with a particle size of 5 µm.
The procedure requires an injection of 10 µL with a flow rate of 1 mL/min.