Agilent 1100 LC/MSD Analysis of LSD and LAMPA Application Note

This document introduces the LC/MS detection method of LSD.

Brand: Agilent
Category: Other
Document type: Application Note
Language: English
Pages: 2
File format: PDF
File size: 156 KB
Published: 03 July, 2012
Updated: 30 September, 2023
MD5 checksum: 949AF20B8BEA665E8AE276C4F0B10E6F
PDF: Agilent 1100 LC/MSD Analysis of LSD and LAMPA Application Note PDF

About this manual

This document details a method for the detection and separation of LSD and its internal standard, LAMPA (lysergic acid methylpropylamide). The method utilizes an Agilent 1100 LC/MSD system equipped with a ZORBAX Eclipse XDB-C8 column to achieve rapid separation of these compounds, which is critical due to the low dosages and rapid excretion of LSD.

What's inside

Application
Highlights
Conditions
Instrument
Column
Mobile Phase
HP 1100 MSD Conditions

Specifications

Column	ZORBAX Eclipse XDB-C8, 2.1 x 50 mm, 5pm
Column Part Number	960967-906
Mobile Phase Additive	10 mM ammonium formate, pH 3.7
Flow Rate	0.3 mL / min
Injection Volume	30 pl
Column Temperature	30°C
Detection Mode	SIM mode
LSD Ion	324.2
LAMPA Ion	223.1
Internal Standard Ion	208.1

Frequently asked questions

What is the internal standard used for LSD analysis?

LAMPA (lysergic acid methylpropylamide) is used as an internal standard because it has the same molecular weight as LSD.

What are the peak volumes for gradient and isocratic separations?

Peak volumes are 150uL for the gradient separation and 200uL for the isocratic separation.

Which column is recommended for this analysis?

The ZORBAX Eclipse XDB-C8 column is recommended for good peak shape over a broad pH range.

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