The document describes the USP method for analyzing Buspirone in pharmaceutical applications using HPLC. It highlights the use of ZORBAX Eclipse XDB-C18 column for improved peak shape and higher efficiency.
This document presents an application comparing the analysis of the anxiolytic drug Buspirone using the original L1 column packing against the modern ZORBAX Eclipse XDB-C18 column. The comparison is based on the United States Pharmacopeia (USP) method for Buspirone HCl.
| Column Part Number | 993967-902 |
|---|---|
| Mobile Phase | ACN : 10 mM KH2PO4 (pH 7.5) (40:60) |
| Flow Rate | 1 mL / min |
| Detection Wavelength | 254 nm |
| Injection Volume | 25 pL |
The standard flow rate for the older 3.9 x 300 mm columns is 2.0 ml/min, though 1.0 ml/min is typical for 150 mm columns.
The standard flow rate for the older 3.9 x 300 mm columns is 2.0 ml/min.
On 150 mm columns, the flow rate is typically 1.0 ml/min.
The ZORBAX Eclipse XDB-C18 column used in the comparison has dimensions of 4.6 x 150 mm.