This document describes the analysis of Nortriptyline in plasma. It utilizes on-line extraction and separation using a ZORBAX Eclipse XDB-C8 column, which provides excellent peak shape. The method operates optimally over a wide pH range. This fast and direct approach is used to determine physiological levels of Nortriptyline in the bloodstream.
This document describes an analytical procedure for determining the level of nortriptyline, a tricyclic antidepressant, in blood serum. The method utilizes on-line sample preparation with column switching to achieve fast and direct analysis.
The separation is performed using a ZORBAX Eclipse XDB-C8 column (Agilent P/N: 993967-906). The mobile phase consists of 28% acetonitrile in 20 mM sodium phosphate buffer at pH 2.8, with detection via UV absorbance at 210 nm.
| Column Part Number | 993967-906 |
|---|---|
| Mobile Phase Composition | 28% ACN in 20 mM sodium phosphate buffer, pH 2.8 |
| Flow Rate | 1.0 ml/min |
| Detection Wavelength | 210 nm |
It is intended for investigational and research purposes only to determine physiological levels upon treatment or misuse.
This procedure is for investigational and research use only and is not for in vitro diagnostic procedures.
The analysis uses a flow rate of 1.0 ml/min.
The UV detector is set to 210 nm.