The document describes a method for analyzing carbamazepine and phenytoin in plasma. The analysis is performed using online extraction and a ZORBAX SB-CN column, allowing for fast and direct analysis of these anticonvulsant drugs.
This document details an analytical procedure for determining the levels of carbamazepine and phenytoin in plasma samples. The method utilizes on-line sample preparation and concentration via column switching to provide a fast and direct approach for clinical settings where these anticonvulsant drugs are assayed. The final separation is achieved using a ZORBAX SB-CN column, which operates optimally at low pH.
| Column | ZORBAX SB-CN, 4.6 x 150 mm, 5um |
|---|---|
| Mobile Phase | 28% ACN in 116 mM sodium phosphate buffer, pH 2.8 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Sample Volume | 200 uL |
This procedure is for Investigational / Research only and is not for in vitro diagnostic procedures.
The analysis uses a ZORBAX SB-CN column.