This document introduces the stability of ZORBAX Eclipse XDB-C8 column to neutral pH conditions, and verifies in experiments that ZORBAX Eclipse XDB-C8 column can be used for a longer time in neutral pH conditions.
This document addresses concerns regarding chromatographic reproducibility caused by column aging. It highlights that while all columns undergo an "aging process" upon use, certain mobile phases can degrade the bonded phase or silica in standard columns.
The text specifically compares a ZORBAX Eclipse XDB-C8 column with a popular C8 column using a pH 7 phosphate buffer mobile phase over 125 days. The comparison demonstrates that the ZORBAX column undergoes minimal change (10%) compared to the competitor's significant shift (over 25% in the first 25 days). This stability is attributed to the ZORBAX column being made of solgel-type silica and featuring double endcapping, which acts as a more effective protective coating against mobile phase corrosion than the xerogel-type silica used by the competitor.
The ZORBAX Eclipse XDB-C8 is highly stable in intermediate pH mobile phases while competitive xerogel-type columns are not.
At mid pH, changes are due to the dissolution of silica and the creation of additional hydroxyl groups, not silane hydrolysis.