Using smaller-diameter columns can improve instrument sensitivity (peak response), save time and reduce solvent consumption
This application note discusses the advantages of modernizing High-Performance Liquid Chromatography (HPLC) methods by utilizing ZORBAX Solvent-Saver or Narrow-Bore columns. The text focuses on a specific USP method for nitrofurantoin HPLC assay, demonstrating how switching to smaller diameter columns improves productivity.
| Original USP Column Dimensions | 3.9 x 300 mm |
|---|---|
| Narrow-Bore Column Dimensions (Example A) | 2.1 x 150 mm |
| Solvent-Saver Column Dimensions (Example B) | 3.0 x 150 mm |
| Analytical Column Dimensions (Example C) | 4.6 x 150 mm |
| Peak Response Increase (Narrow-Bore vs Analytical) | 3 times greater |
| Mobile Phase Savings (Narrow-Bore vs Analytical) | 40% savings |
Using smaller-diameter columns offers increased instrument sensitivity (peak response), savings in analysis time, and reduced solvent consumption.
The peak response for the 2.1 mm id. column is 3 times greater than that of the 4.6 mm id. column.
The shift is due to extra-column volume, which reduces column efficiency especially for early eluting peaks.