Agilent Effect of Bonded-Phase Functionality on the Recovery of a Synthetic Lipopeptide Application Note

About this manual

This document presents an application study by Robert Ricker regarding the separation and recovery of a highly hydrophobic synthetic lipopeptide. The study compares different StableBond bonded phases to determine which offers the best peptide recovery under specific conditions.

The results indicate that more hydrophobic, long-chain alkyl bonded-phases (specifically SB-C8 and SB-C18) yield higher recovery rates compared to polar phases like SB-CN. This is attributed to higher organic solvent concentrations in the mobile phase at elution, which improves peptide solubility despite being counter-intuitive to conventional wisdom.

What's inside

• Effect of Bonded-Phase Functionality
• Recovery of Synthetic Lipopeptide
• StableBond bonded phases
• High temperature separation
• Mobile phase conditions
• Peptide solubility

Frequently asked questions

What recovery percentage is achieved using the SB-C18 bonded phase?

The SB-C18 bonded phase achieves a recovery of 89%.

What temperature is required for the separation of this lipopeptide?

High temperature is required for the separation of this highly hydrophobic lipopeptide.

What is the column inner diameter used in the described separation conditions?

The separation conditions use a column with an inner diameter of 4.6 mm.