This application note describes the methods for measuring protein size and protein expression using the Agilent 2100 Bioanalyzer and Protein 200 LabChip® kit.
This document describes the performance of the Agilent Protein 200 LabChip kit when used with the Agilent 2100 Bioanalyzer system. It compares the kit's capabilities against traditional SDS-polyacrylamide gel electrophoresis (SDS-PAGE) methods.
| Resolution | at least 10 % |
|---|---|
| Detection limit | 20 ng/pl |
| Linear dynamic range | 20 ng/pl to 2000 ng/pl |
| Analysis time | less than 30 minutes |
The assay sizes and analyzes proteins ranging in size from 14-200 kD under denaturing conditions.
The sensitivity is comparable to standard Coomassie stain, with a lower detection limit of 20 ng/pl BSA.
The assay is linear over two orders of magnitude, specifically from 20 ng/pl to 2000 ng/pl BSA in PBS.