This document describes the development of a rapid resolution liquid chromatography (RRLC) method for the analysis of notoginseng. The method has good resolution and can be used to quickly analyze complex components, thereby increasing sample throughput and lowering costs. The method can also be used to analyze compounds in different parts of the notoginseng plant, and different peak profiles and different concentrations of certain saponins are detected.
This document details an Application Note by Zhi-xiu Xu regarding the analysis of notoginseng using Agilent's Rapid Resolution Liquid Chromatography (RRLC). The study focuses on transferring a conventional High-Performance Liquid Chromatography (HPLC) method to RRLC technology to achieve shorter run times while maintaining or improving resolution for complex Traditional Chinese Medicine samples.
An Agilent 1200 Series Rapid Resolution LC system consisting of a binary pump, autosampler, column compartment, diode array detector, and ChemStation software.
An Agilent ZORBAX SB C18 column, 4.6 x 50 mm, 5 pm particle size.
The typical run time is longer than 60 minutes.