A rapid, simple, highly sensitive procedure for the simultaneous analysis of 14 benzodiazepines and six metabolites in urine and blood, using the Agilent 6410 Triple Quadrupole Mass Spectrometer in electrospray mode, is described.
The document outlines a forensic procedure for the simultaneous analysis of benzodiazepines and their metabolites in urine and blood samples. The method utilizes the Agilent 6410 Triple Quadrupole Mass Spectrometer in electrospray mode, featuring unique software capabilities to monitor qualifying ions and calculate ion ratios for result confirmation.
Detailed sample preparation protocols are provided for both urine and blood, including specific extraction and cleanup steps. The analytical procedure specifies the use of an Agilent 1200 Series LC pump and a ZORBAX Eclipse XDB C18 column, with distinct mass spectrometric parameters for the parent benzodiazepines and their 7-amino metabolites.
| Instrument | Agilent 6410 LC Triple Quadrupole Mass Spectrometer |
|---|---|
| LC Pump | Agilent 1200 Series LC pump |
| Column | ZORBAX Eclipse XDB C18 4.6 x 50 mm ~ 1.8-um |
| Operation Mode | Electrospray positive mode |
| Gas Temperature (7-Amino) | 350°C |
| Capillary Voltage (7-Amino) | 4000 V |
The procedure analyzes 14 benzodiazepines and six metabolites simultaneously.
The solvent flow rate for the 7-amino metabolites method is 0.6 mL/min.
The column temperature for the 7-amino metabolites method is 45°C.