Optimizing the Agilent 1100 Series well-plate sampler
The document details optimization strategies for the Agilent 1100 Series well-plate sampler used in HPLC systems. It focuses on minimizing carry-over through specific needle wash procedures and injector programs that utilize valve bypass positions to clean internal channels.
Additionally, the note covers methods to achieve fast injection cycles by utilizing overlapped injection modes and reducing system delay volume. Performance data regarding linearity, area precision, and cycle times are presented alongside experimental results for various compounds.
The autosampler is able to draw and inject a sample within 36 seconds, including an outside needle wash of 10 seconds.
The injector program switches the injection valve into the bypass position at the end of equilibration to clean the bypass channel and main channel.
Switching the injection valve into the bypass position reduces the system delay volume by approximately 300 pl.