Label-free analysis of microsatellite instability in colorectal carcinoma by on-chip electrophoresis

About this manual

The text describes a research study aimed at developing a simple and efficient procedure for Microsatellite Instability (MSI) detection, an important tool in clinical diagnostics for various cancers including colorectal carcinoma. The study compares conventional techniques like polyacrylamide gel electrophoresis with a new method using microfluidic on-chip electrophoresis.

What's inside

• Abstract
• Introduction
• Material and Methods
• DNA extraction and PCR
• MSI analysis by fluorescence-linked PCR
• MSI analysis by on-chip electrophoresis
• Results and Discussion
• High resolution of the amplified microsatellite loci

Frequently asked questions

How long does the label-free analysis of microsatellite amplicons take using this method?

The analysis allows for label-free analyses within 30 minutes.

Which five microsatellite loci were analyzed in the study?

The recommended set comprises Bat25, Bat26, D2S123, D5S346, and D17S250.