In this Application Note, the Agilent 6140 quadrupole MS, equipped with the multimode source, is combined with the Agilent 1200 Series Rapid Resolution LC system to develop a fast, generic LC/MS method for the analysis of 35 pharmaceutical compounds from 17 compound classes, reducing the method run time from 2.1 to 0.8 minutes.
This document details the development of a rapid Liquid Chromatography-Mass Spectrometry (LC-MS) method designed for high-throughput analysis in drug discovery. The study combines the Agilent 6140 quadrupole MS with multimode source and the Agilent 1200 Series Rapid Resolution LC system to achieve run times of less than one minute while maintaining resolution.
The authors present a generic method optimized for analyzing 35 pharmaceutical compounds from various classes. Key optimizations include using a mixed-mode ionization source, increasing flow rates up to 1.6 mL/min, reducing delay volume via ChemStation software features, and utilizing high-temperature columns (up to 90 °C) with sub-2-micron particle technology.
The method achieves a reduction of the run time to less than 1 minute (specifically 0.8 minutes).
The flow rate was increased to 1.6 mL/min.