This document introduces the ZORBAX Extend-C18 columns from Agilent Technologies, which can be used for separating explosive compounds. The columns offer various configurations for customized HPLC methods based on resolution, speed, and solvent usage. The document also highlights the advantages of different column configurations, including resolution, speed, and solvent savings. By simply substituting the column, users can choose the desired combination of resolution, speed, and solvent usage.
This document presents a study on using Agilent ZORBAX Extend-C18 columns to separate explosive compounds specified in EPA method 8330. The authors demonstrate that various column configurations allow for customized High-Performance Liquid Chromatography (HPLC) methods tailored to specific needs regarding resolution, analysis speed, and solvent consumption.
The text details experimental results using different column dimensions and particle sizes (1.8 µm and 3.5 µm) to achieve baseline resolution for 14 nitroaromatic and nitramine compounds. It compares the performance of a high-throughput 1.8 µm column against a standard 5 µm column and a solvent-saving 3.0 mm inner diameter column, highlighting trade-offs in analysis time, system pressure, and solvent usage.
| Column Type | Extend-C18 |
|---|---|
| Particle Size Options | 1.8 µm, 3.5 µm, 5 µm |
| Mobile Phase A | 5 mM ammonium formate in water |
| Mobile Phase B | Methanol |
| Temperature | 41°C |
| Flow Rate (RRHT) | 1.7 mL/min |
| Flow Rate (Solvent Saver) | 1.1 mL/min |
The study uses ZORBAX Extend-C18 columns with Rapid Resolution High Throughput (RRHT) and Solvent Saver Plus configurations.
The 1.8 µm column reduces analysis time and provides high resolution, though it generates higher back pressure.
It reduces solvent consumption by half while maintaining similar retention and resolution.