This is an application note describing a method for quantification of digoxin using liquid chromatography-mass spectrometry (LC-MS) in human serum
The document details an analytical method for determining digoxin levels in human serum. It addresses the limitations of traditional immunoassays and HPLC methods, such as lack of specificity or insufficient sensitivity without derivatization.
The proposed solution utilizes Liquid Chromatography/Mass Spectrometry (LC/MS) with a specific online sample preparation technique. This method employs a precolumn packed with LiChrospher ADS RP4 to remove matrix proteins and concentrate the analyte, followed by separation on a Hypersil SAS column. The note provides experimental conditions for both APCI and ESI ionization sources.
The therapeutic range for digoxin is 0.5 to 2.0 ng/ml.
The limit of quantification is 0.5 ng digoxin per ml of human serum.