A fast, sensitive and reproducible technique for confirming the presence of drugs of abuse (DOA) in oral fluids (OF) using the Agilent G6410AA Triple Quadrupole Mass Spectrometer (QQQ) is presented.
This document presents a fast, sensitive, and reproducible technique for confirming the presence of drugs of abuse (DOA) in oral fluids (OF) using the Agilent G6410AA Triple Quadrupole Mass Spectrometer. The method utilizes liquid chromatography/tandem mass spectrometry (LC/MS/MS) with a 1.8-µm, C18 column to achieve high chromatographic resolution with elution times of less than 4.2 minutes for THC and less than 1.5 minutes for other analyzed drugs. The technique meets the sensitivity requirements set by the United States Substance Abuse and Mental Health Services Administration (SAMHSA) for workplace testing.
| Column temperature | 40°C |
|---|---|
| Flow rate | 0.5 mL/min |
| Elution time (THC) | Less than 4.2 minutes |
| Elution time (Cocaine, Amphetamine, Methamphetamine, MDMA) | Less than 1.5 minutes |
The elution time for THC is less than 4.2 minutes.
The elution time for these drugs is less than 1.5 minutes.
The drugs analyzed include THC, cocaine, amphetamine, methamphetamine, and MDMA.