This document describes the polarity switching technology in mass spectrometric analysis and the ability of a mass spectrometer to switch polarities during a single chromatographic peak. This capability allows for the detection of various polarity molecules in unknown samples during a single run, saving time and improving data quality.
This document describes the capabilities of the Agilent 1100 Series LC/MSD Trap SL ion trap mass spectrometer for analyzing samples containing compounds that favor both positive and negative ionization modes. It highlights the instrument's ability to switch polarities rapidly (approximately 0.5 seconds) to acquire data from unknown analytes in a single chromatographic run.
| Polarity switching time | ~ 0.5 seconds |
|---|---|
| Column temperature | 40°C |
| Flow rate | 0.4 ml/min |
| Injection volume | 3 pl |
| Capillary voltage | 3000 V |
| Scan range | 50-600 m/z |
The instrument features extremely fast polarity switching of approximately 0.5 seconds.
Acetone was added to the mobile phase to provide thermal electrons for negative mode APPI.