This Application Note will demonstrate the use of the Agilent 1200 Series Rapid Resolution LC system with Rapid Resolution High Throughput (RRHT) columns for the separation of the ingredients found in a complex ginseng root extract. The information obtained with an ion trap MSn and MRM is used to determine the structure of the ingredients and for quality control purposes.
This document describes an analytical method using the Agilent 1200 Series Rapid Resolution LC system coupled with an Agilent 6330 Ion Trap LC/MS to analyze a complex extract from Asian ginseng root (Panax ginseng). The primary goal is to separate the ingredients and determine their structures for quality control purposes.
The study details the experimental setup, including the specific hardware components like the binary pump, autosampler, thermostatted column compartment, and diode array detector. It outlines the chromatographic conditions, such as the gradient profile and flow rate, as well as the mass spectrometry parameters for ESI source operation and ion trap scanning. The results show the successful separation of major ginsenosides (Re, Rf, F1, Rb1, Rb2, Rc, Rd) and the use of MS/MS and MS3 fragmentation data to elucidate their structures.
| Column particle size | 1.8 µm |
|---|---|
| Flow rate | 0.5 mL/min |
| Scan speed | 26,000 m/z/sec |
| Sample injection volume | 2 µL |
| Column temperature | 50 °C |
| Dry gas temperature | 300 °C |
| Scan range | 200-1300 m/z |
The binary pump was operated with a flow rate of 0.5 mL/min.
The samples were cooled to 10°C in the autosampler.
The ion trap is able to scan with a speed of 26,000 m/z/sec.