This is an application note that describes a protein profiling approach that combines accurate mass ESI-TOF data with differential analysis to highlight and identify putative biomarkers.
This document presents a protein profiling approach combined with differential analysis to identify putative biomarkers. The method utilizes accurate mass ESI-TOF data for rapid differential expression analysis, followed by profile-directed identification from MS/MS data. This approach is designed to highlight low-abundance proteins that might be missed by traditional data-dependent acquisition strategies.
| Enrichment column volume | 40-nL |
|---|---|
| Analytical column dimensions | 150 mm x 75 pm |
| Column material | Zorbax 300SB-C18, 5 pm |
| Gradient method duration | 100-minute |
| Analytical flow rate | 300 nL/min |
| Sample loading flow rate | 4 pL/min |
The approach uses an Agilent 1200 Series HPLC-Chip/MS system interfaced to an Agilent 6210 TOF mass spectrometer.
Mass Profiler software is used for feature extraction and differential analysis of the complex data sets.