This paper introduces a method for optimizing the separation of organic acids in fruit juice using low-pH ZORBAX Stable-Bond SB-Aq columns. The use of short columns, little organic solvent, and low-pH mobile phase enables rapid separation of organic acids in fruit juice.
This document describes a chromatographic method for separating salicylic acid and its major impurities. It evaluates the effect of different acid additives—trifluoroacetic acid (TFA), phosphoric acid, acetic acid, and formic acid—on the selectivity and peak shape of the separation. The study utilizes Agilent's Rapid Resolution LC (RRLC) system equipped with StableBond SB-Aq columns to achieve high-throughput analysis.
| Column Part Number | 827975-914 |
|---|---|
| Column Dimensions | 4.6 mm x 50 mm |
| Particle Size | 1.8 um |
| Detection Wavelength | 220 nm |
The document tested trifluoroacetic acid (TFA), phosphoric acid, acetic acid, and formic acid as mobile-phase additives.
TFA and phosphoric acid yielded the best separations, while formic acid and acetic acid resolved only five of six components.