This application note demonstrates the performance of the Agilent 1290 Infinity LC System coupled to an Agilent 6460A Triple Quadrupole LC/MS for the evaluation of metabolic stability using the cassette analysis approach combined with fast polarity switching.
The document presents a UHPLC/MS/MS method that achieves high throughput analysis with retention times less than 0.8 minutes at a flow rate of 1.5 mL/min and binary pump pressure of 1,100 bar. It utilizes the Agilent 1290 Infinity LC System and an Agilent 6460A Triple Quadrupole LC/MS System with Jet Stream technology to monitor six transitions while switching polarity in just 30 ms.
| Flow rate | 1.5 mL/min |
|---|---|
| Binary pump pressure | 1,100 bar |
| MS cycle time | 114 ms |
| Polarity switching time | 30 ms |
| Data points per peak | at least 9 points |
| Accuracy range | 90.7 — 107.1% |
The polarity switching time is 30 ms.
At least 9 points are collected across the chromatographic peaks.
The accuracy over the expected concentration range is between 90.7% and 107.1%.